Leptin directly stimulates aromatase activity in human luteinized granulosa cells.

P-200: Effects of The ?3 Fatty Acid Eicosapentaenoic Acid on PPARa and Aromatase in Human Granulosa Cells

Leptin suppresses anti-Mullerian hormone gene expression through the JAK2/STAT3 pathway in luteinized granulosa cells of women undergoing IVF.

STUDY QUESTION Do the adipocytokines, leptin and adiponectin affect the granulosa cell expression of anti-Mullerian hormone (AMH) and its receptor (AMHR-II)? SUMMARY ANSWER Leptin suppresses AMH mRNA levels in human luteinized granulosa cells through the JAK2/STAT3 pathway, while adiponectin has no such effect. WHAT IS KNOWN ALREADY AMH is one of the most reliable markers of ovarian reserve...

Leptin inhibits gonadotrophin-stimulated granulosa cell progesterone production by antagonizing insulin action.

Recent evidence has demonstrated that expression of leptin and leptin receptors is expected in the human ovary, and that leptin alters ovarian steroidogenesis in animal models. This study was designed to determine whether leptin modulates basal, gonadotrophin-, and insulin-stimulated progesterone production by human luteinized granulosa cells (GC). GC were recovered from follicular aspirates ob...

P-227: Functional Analysis of The I.a,I.b, I.c and I.d (PII) Promoters of CYP19 (aromatase) Gene in Granulosa Cells of Polycystic Ovaries Patients and The Role of Letrozole and Antisensearom on CYP19 Gene Expression Inhibition

Background: The key enzyme of estrogen biosynthesis, aromatase cytochrome P450,is encoded by the CYP19 gene.CYP19 plays an important role in the development,function,an regulation of the female reproduction cycle. Thus, it is the potential candidate gene affecting fertility performance in human. CYP19 transcripts are expressed mainly in the ovary,testes,breast,adipose tissue and brain. Tissue e...

Oestradiol production by luteinized human granulosa cells: evidence of the stimulatory action of recombinant human follicle stimulating hormone.

In this study the effect of recombinant human follicle stimulating hormone (rFSH) on oestradiol production by human granulosa-lutein cells was examined in long-term culture, in the presence or absence of androgens. Cells were harvested at the time of follicular aspiration after ovarian hyperstimulation for in-vitro fertilization and cultured for 9 days. Granulosa cells were capable of secreting...